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Detection of inflammatory cytokines in skin wash of healthy volunteers and patients with AD and psoriasis. Cytokine concentrations were measured in skin wash fluid using Luminex assay. Inflammatory cytokines ( a <t>)</t> <t>IL-1β,</t> ( b ) <t>TNFα,</t> ( c ) IL-18, and ( d ) IL-8. AD-related immune mediators ( e ) CCL13, ( f ) CCL18, ( g ) sIL-2R, and ( h ) IL-4. Psoriasis-associated immune mediators ( i ) IL-17A, ( j ) IL-23, ( k ) sTNFR1, and ( l ) IFNγ. Concentrations are depicted as mean ± SD, with individual data points shown. PS denotes psoriasis, NL denotes nonlesional, and L denotes lesional. ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .001. AD, atopic dermatitis; HC, healthy control; sIL-2R, soluble IL-2R; sTNFR1, soluble TNFR1.
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Detection of inflammatory cytokines in skin wash of healthy volunteers and patients with AD and psoriasis. Cytokine concentrations were measured in skin wash fluid using Luminex assay. Inflammatory cytokines ( a <t>)</t> <t>IL-1β,</t> ( b ) <t>TNFα,</t> ( c ) IL-18, and ( d ) IL-8. AD-related immune mediators ( e ) CCL13, ( f ) CCL18, ( g ) sIL-2R, and ( h ) IL-4. Psoriasis-associated immune mediators ( i ) IL-17A, ( j ) IL-23, ( k ) sTNFR1, and ( l ) IFNγ. Concentrations are depicted as mean ± SD, with individual data points shown. PS denotes psoriasis, NL denotes nonlesional, and L denotes lesional. ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .001. AD, atopic dermatitis; HC, healthy control; sIL-2R, soluble IL-2R; sTNFR1, soluble TNFR1.
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Detection of inflammatory cytokines in skin wash of healthy volunteers and patients with AD and psoriasis. Cytokine concentrations were measured in skin wash fluid using Luminex assay. Inflammatory cytokines ( a <t>)</t> <t>IL-1β,</t> ( b ) <t>TNFα,</t> ( c ) IL-18, and ( d ) IL-8. AD-related immune mediators ( e ) CCL13, ( f ) CCL18, ( g ) sIL-2R, and ( h ) IL-4. Psoriasis-associated immune mediators ( i ) IL-17A, ( j ) IL-23, ( k ) sTNFR1, and ( l ) IFNγ. Concentrations are depicted as mean ± SD, with individual data points shown. PS denotes psoriasis, NL denotes nonlesional, and L denotes lesional. ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .001. AD, atopic dermatitis; HC, healthy control; sIL-2R, soluble IL-2R; sTNFR1, soluble TNFR1.
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Detection of inflammatory cytokines in skin wash of healthy volunteers and patients with AD and psoriasis. Cytokine concentrations were measured in skin wash fluid using Luminex assay. Inflammatory cytokines ( a <t>)</t> <t>IL-1β,</t> ( b ) <t>TNFα,</t> ( c ) IL-18, and ( d ) IL-8. AD-related immune mediators ( e ) CCL13, ( f ) CCL18, ( g ) sIL-2R, and ( h ) IL-4. Psoriasis-associated immune mediators ( i ) IL-17A, ( j ) IL-23, ( k ) sTNFR1, and ( l ) IFNγ. Concentrations are depicted as mean ± SD, with individual data points shown. PS denotes psoriasis, NL denotes nonlesional, and L denotes lesional. ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .001. AD, atopic dermatitis; HC, healthy control; sIL-2R, soluble IL-2R; sTNFR1, soluble TNFR1.
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Detection of inflammatory cytokines in skin wash of healthy volunteers and patients with AD and psoriasis. Cytokine concentrations were measured in skin wash fluid using Luminex assay. Inflammatory cytokines ( a <t>)</t> <t>IL-1β,</t> ( b ) <t>TNFα,</t> ( c ) IL-18, and ( d ) IL-8. AD-related immune mediators ( e ) CCL13, ( f ) CCL18, ( g ) sIL-2R, and ( h ) IL-4. Psoriasis-associated immune mediators ( i ) IL-17A, ( j ) IL-23, ( k ) sTNFR1, and ( l ) IFNγ. Concentrations are depicted as mean ± SD, with individual data points shown. PS denotes psoriasis, NL denotes nonlesional, and L denotes lesional. ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .001. AD, atopic dermatitis; HC, healthy control; sIL-2R, soluble IL-2R; sTNFR1, soluble TNFR1.
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Effects on IL-1β (A) , IL-6 (B) , and IL-8 (C) levels in HFLS-RA cells induced <t>by</t> <t>TNF-α</t> (5 ng/mL). n = 3; vs. 0 h, ** p < 0.01; **** p < 0.0001.
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Effects on IL-1β (A) , IL-6 (B) , and IL-8 (C) levels in HFLS-RA cells induced <t>by</t> <t>TNF-α</t> (5 ng/mL). n = 3; vs. 0 h, ** p < 0.01; **** p < 0.0001.
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Effects on IL-1β (A) , IL-6 (B) , and IL-8 (C) levels in HFLS-RA cells induced <t>by</t> <t>TNF-α</t> (5 ng/mL). n = 3; vs. 0 h, ** p < 0.01; **** p < 0.0001.
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Detection of inflammatory cytokines in skin wash of healthy volunteers and patients with AD and psoriasis. Cytokine concentrations were measured in skin wash fluid using Luminex assay. Inflammatory cytokines ( a ) IL-1β, ( b ) TNFα, ( c ) IL-18, and ( d ) IL-8. AD-related immune mediators ( e ) CCL13, ( f ) CCL18, ( g ) sIL-2R, and ( h ) IL-4. Psoriasis-associated immune mediators ( i ) IL-17A, ( j ) IL-23, ( k ) sTNFR1, and ( l ) IFNγ. Concentrations are depicted as mean ± SD, with individual data points shown. PS denotes psoriasis, NL denotes nonlesional, and L denotes lesional. ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .001. AD, atopic dermatitis; HC, healthy control; sIL-2R, soluble IL-2R; sTNFR1, soluble TNFR1.

Journal: JID Innovations

Article Title: Development of a noninvasive device and method for measuring immune parameters in skin disease

doi: 10.1016/j.xjidi.2026.100456

Figure Lengend Snippet: Detection of inflammatory cytokines in skin wash of healthy volunteers and patients with AD and psoriasis. Cytokine concentrations were measured in skin wash fluid using Luminex assay. Inflammatory cytokines ( a ) IL-1β, ( b ) TNFα, ( c ) IL-18, and ( d ) IL-8. AD-related immune mediators ( e ) CCL13, ( f ) CCL18, ( g ) sIL-2R, and ( h ) IL-4. Psoriasis-associated immune mediators ( i ) IL-17A, ( j ) IL-23, ( k ) sTNFR1, and ( l ) IFNγ. Concentrations are depicted as mean ± SD, with individual data points shown. PS denotes psoriasis, NL denotes nonlesional, and L denotes lesional. ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .001. AD, atopic dermatitis; HC, healthy control; sIL-2R, soluble IL-2R; sTNFR1, soluble TNFR1.

Article Snippet: E coli –derived recombinant human TNFα, IL-1β, IL-6, IL-8, IL-10, and IL-18 (endotoxin levels <0.10 EU/μg by the limulus amebocyte lysate method) were purchased from R&D Systems and reconstituted in PBS.

Techniques: Luminex, Control

Effects on IL-1β (A) , IL-6 (B) , and IL-8 (C) levels in HFLS-RA cells induced by TNF-α (5 ng/mL). n = 3; vs. 0 h, ** p < 0.01; **** p < 0.0001.

Journal: Frontiers in Medicine

Article Title: Identification of diagnostic genes in rheumatoid arthritis using integrated bioinformatics, machine learning, and experimental validation

doi: 10.3389/fmed.2026.1803764

Figure Lengend Snippet: Effects on IL-1β (A) , IL-6 (B) , and IL-8 (C) levels in HFLS-RA cells induced by TNF-α (5 ng/mL). n = 3; vs. 0 h, ** p < 0.01; **** p < 0.0001.

Article Snippet: Upon reaching approximately 70% confluence, cells were stimulated with 5 ng/mL recombinant human TNF-α (Servicebio, China) to establish an in vitro rheumatoid arthritis model.

Techniques:

Quantitative analysis of MAGED1 (A) , EPYC (B) , and LAP3 (C) mRNA expression in HFLS-RA induced by TNF-α (5 ng/mL). n = 3; vs. 0 h, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Frontiers in Medicine

Article Title: Identification of diagnostic genes in rheumatoid arthritis using integrated bioinformatics, machine learning, and experimental validation

doi: 10.3389/fmed.2026.1803764

Figure Lengend Snippet: Quantitative analysis of MAGED1 (A) , EPYC (B) , and LAP3 (C) mRNA expression in HFLS-RA induced by TNF-α (5 ng/mL). n = 3; vs. 0 h, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Upon reaching approximately 70% confluence, cells were stimulated with 5 ng/mL recombinant human TNF-α (Servicebio, China) to establish an in vitro rheumatoid arthritis model.

Techniques: Expressing